Malaria rapid diagnostic test procedure

CDC - Malaria - Diagnostic Tool

  1. ed with a 100X oil immersion objective. Visual criteria are used to detect malaria parasites and to differentiate (when possible) the various species
  2. Malaria rapid diagnostic tests (RDTs) assist in the diagnosis of malaria by detecting evidence of malaria parasites (antigens) in human blood. RDTs permit a reliable detection of malaria infections particularly in remote areas with limited access to good quality microscopy services. This site provides information and guidance to malaria control.
  3. Malaria diagnosis with Rapid Diagnostic Tests (RDTs) is a clinical service and as such Standard Operating Procedures (SOPs) are required to guide private sector providers. These SOPs for RDTs provide a standardized procedure to perform RDTs as agreed by private providers, trainers and supervisors
  4. 10 The Use of Malaria RDTs Malaria Rapid Diagnostic Tests 11 immediately available (e.g. remote travellers), a plan of action should be prepared beforehand in consultation with an appropriate health worker
  5. utes) which helps in starting the treatment quickly

Clinical findings should always be confirmed by a laboratory test for malaria. In addition to ordering the malaria specific diagnostic tests described below, the health-care provider should conduct an initial workup and request a complete blood count and a routine chemistry panel Rapid diagnostic test for malaria parasite is done by using the method of the lateral-flow immunochromatographic assay (Dipstick Technology). The immunochromatographic assay relies on the migration of liquid or blood across the surface of a nitrocellulose membrane test strip which has been impregnated with antibodies Rapid diagnostic tests or RDTs for malaria are a way to test whether a person with malaria like symptoms actually has malaria. Malaria parasites produce proteins called antigens. RDTs detect these malarial antigens in a person's blood. If malaria antigens are present, the test will be positive

The BinaxNOW ® Malaria test is an in vitro immunochromatographic assay for the qualitative detection of Plasmodium antigens circulating in human venous and capillary EDTA whole blood of individuals with signs and symptoms of malarial infection Giemsa microscopy and rapid diagnostic tests (RDTs) represent the two diagnostics most likely to have the largest impact on malaria control today. These two methods, each with characteristic strengths and limitations, together represent the best hope for accurate diagnosis as a key component of successful malaria control The rapid diagnostic tests (RDTs) whose main interest lies in their implementation without special equipment by unskilled personnel have grown significantly over the past fifteen years to diagnose malaria. They rely on the detection of specific Plasmodium proteins, PfHRP2, pLDH and aldolase. If the Of the 852 samples, 103 tested positive for malaria by any method, of which 95 were positive by PCR. Of these 95 samples, malaria was identified by microscopy in 81 (85%), compared with 92 (97%) by the rapid diagnostic test [ 20 ]. The rapid diagnostic test had a negative predictive value of 99.6%, compared with 98.2% for microscopy

Malaria Rapid Diagnostic Tests (RDTs) are used increasingly for diagnosis of malaria, particularly in remote tropical areas where good microscopy-based diagnosis is impractical. RDTs must therefore be robust, simple and safe to use, and reliably demonstrate when malaria parasitaemia is, and is not, present Malaria Rapid Diagnostic Tests (RDTs) are widely used for diagnosing malaria. The present retrospective study evaluated the CareStart™ Malaria HRP-2/pLDH (Pf/pan) Combo Test targeting the Plasmodium falciparum specific antigen histidine-rich protein (HRP-2) and the pan- Plasmodium antigen lactate dehydrogenase (pLDH) in a reference setting In parallel to this, accurate and affordable rapid diagnostic tests for malaria (RDTs) have become available over the last decade and the 2010 WHO malaria treatment guidelines now propose that wherever possible all patients suspected of malaria should be tested by blood slide or RDT and only those with a positive test result should receive anti. Malaria rapid diagnostic tests (RDTs) assist in the diagnosis of malaria by detecting evidence of malaria parasites (antigens) in human blood. These tests require a drop of peripheral blood, normally collected from a finger or heel prick. Visual read-outs are available typically within 20 mins or less

WHO Malaria rapid diagnostic test

Combination rapid diagnostic test Test for detecting multiple malaria species and which distinguishes P. falciparum from other malaria species as a combo test Component Dedicated parts of a finished, packaged, labelled RDT kit that are specific to and necessary for performing the RDT Malaria is a serious condition in the non-immune traveller, and prognosis depends on timely diagnosis. Although microscopy remains the cornerstone of diagnosis, malaria rapid diagnostic tests (RDTs) are increasingly used in non-endemic settings. They are easy to use, provide results rapidly and require no specific training and equipment rapid diagnostic tests (RDT) available in Nigerian market alongside with traditional microscopy. Materials and methods. 525 samples of patients that presented with acute uncomplicated malaria through clinical diagnosis were evaluated with the various tests. Total WBC count and haematocrit were also measured. Results Malaria rapid diagnostic tests (RDTs) emerged in the early 1990s into largely unregulated markets, and uncertain field performance was a major concern for the acceptance of tests for malaria case management. This, combined with the need to guide procurement decisions of UN agencies and WHO Member States, led to the creation of an independent, internationally coordinated RDT evaluation. Rapid diagnostic tests for malaria (RDT) offer a good alternative with the advantage that it is an easy and rapid method, which requires minimum training [6,7,10-12]. The evaluation of different RDTs in many places in the last decade has demonstrated high sensitivity and specificity, compared with expert microscopy diagnosis as gold standard.

Standard Operating Procedures for Malaria Rapid Diagnostic

  1. The Rapid Malaria Tests: The RDTs have been developed in different test formats like the dipstick, strip, card, pad, well, or cassette; and the latter has provided a more satisfactory device for safety and manipulation. The test procedure varies between the test kits
  2. Malaria RDTs are lateral flow immuno-chromatographic antigen-detection tests to detect specific antigens produced by malaria parasites in the blood (commonly obtained from a finger-prick) of infected individuals [ 8 ]
  3. Hence, microscopy of thick and thin film remains the gold standard. Rapid diagnostic tests have acceptable sensitivity and specificity. 4. Discussion. The present study was designed to evaluate the utility in terms of efficiency of existing routine malaria diagnostic tests compared with gold standard microscopy and PCR as internal quality control
  4. ation of the blood smear but in the recent past, rapid diagnostic tests detecting malarial parasite antigens were developed to acquire the result in a lesser amount of time

Rapid diagnostic tests are serological tests that are used in preliminary medical screening or incase of emergency diagnosis. They either detect antibody (Ab) or antigen (Ag) that an individual develops against the infection or the viral protein i.e, the antigen of the pathogen. There are two types of rapid diagnostic test So your test might say you don't have malaria even if you do. For that reason, you may need your blood drawn several times over 2-3 days for the best results. Rapid diagnostic test

Growth Direct System fully automates and accelerates compendial QC Micro testing methods. Automates testing for Environmental Monitoring, Bioburden, and Water Testing Rapid diagnostic tests (RDTs) Malaria rapid diagnostic tests (RDTs) assist in the diagnosis of malaria by detecting evidence of malaria parasites (antigens) in human blood. These tests require a drop of peripheral blood, normally collected from a finger or heel prick. Visual read-outs are available typically within 20 mins or less Rapid diagnostic tests allow us to screen for malaria. These tests are conducted using filter paper strips that contain three types of antibodies—anti-falciparum malaria antibodies, general anti-malaria antibodies, and anti-human antibodies. To test for malaria place serum from a patient on one of these filter paper strips, and allow it to.

One-step Malaria Rapid Test / Malaria Rapid Diagnostic

Malaria Test Procedure, Preparation, Principle, Results

Thus, as with other rapid diagnostic tests, the cost-effectiveness of the test may depend less on the test per se and more on how it is used and what actions are taken based on the results of the test. 18 This will be of particular concern as malaria control efforts become more successful, potentially resulting in more persons with low-level. Background. Malaria is a widespread and life-threatening disease. Each year, 10,000 malaria cases are reported among returned international travellers, and the real number of cases is estimated at 30,000 [].Prompt diagnosis is essential for the treatment and outcome, and malaria rapid diagnostic tests (malaria RDTs) may be of help to non-experienced laboratory staff in non-endemic settings [2,3]

It was recommended that malaria rapid diagnostic tests (RDTs) should be available in all epidemiological situations. But evidence was limited on the implementation of RDTs and its effectiveness in malaria elimination settings. This study examined the implementation of RDTs and how it affected the diagnosis of imported malaria patients in Jiangsu Province, China Malaria antibody detection for clinical diagnosis is performed using the indirect fluorescent antibody (IFA) test. The IFA procedure can be used as a diagnostic tool to determine if a patient has been infected with Plasmodium . Because of the time required for development of antibody and also the persistence of antibodies, serologic testing is. Malaria rapid diagnostic tests (RDT) are widely used in endemic areas in order to comply with the recommendation that malaria treatment should only be given after the clinical diagnosis has been confirmed by RDT or microscopy. However, the overestimation of malaria infection with the use of PfHRP2 based RDT, makes the management of febrile illnesses more challenging

This July, HSL welcomed the World Health Organisation (WHO) malaria rapid diagnostic test (RDT) evaluation programme steering committee to the Halo. Professor Peter Chiodini, consultant speciality lead for parasitology at HSL, is a member of the international committee which oversees the technical and logistical aspects of the global evaluation programme. Chaired by Prof Chiodini, the [ Malaria Rapid Diagnostic Kits (RTDs) are immonochromatographic test kits made simple that can appear as dipstick or cassetes (formats) used in the rapid diagnosis of malaria infection especially in areas of high endemicity. Malaria infection is a major public health challenge in this part of the world The development of rapid and specific diagnostic tests to identify individuals infected with malaria is of paramount importance in efforts to control the severe public health impact of this disease. This study evaluated the ability of a newly developed rapid malaria diagnostic test, OptiMAL (Flow Inc., Portland, Oreg.), to detect Plasmodium vivax and Plasmodium falciparum malaria during an.

Malaria rapid diagnostic tests (RDTs) are instrument-free tests that provide results within 20 min and can be used by community health workers. RDTs detect antigens produced by the Plasmodium parasite such as Plasmodium falciparum histidine-rich protein-2 (PfHPR2) and Plasmodium lactate dehydrogenase (pLDH). The accuracy of RDTs for the diagnosis of uncomplicated P. falciparum infection is. Rapid diagnostic tests for malaria. Rapid diagnostic tests (RDTs) for malaria employ specific antibodies that detect malaria antigens in the blood of infected individuals. RDTs use small blood samples obtained by finger prick or by venepunture and employ a 'lateral diffusion' system similar to a pregnancy test to generate results Individuals infected with malaria are generally very ill with high fevers, shaking chills, and flu-like symptoms and malaise. The most commonly used tests to detect the malaria parasite are: Malarial Parasite by Smear Examination (Blood) Rapid Diagnostic Test (RDT) such as. Malaria detection by Quantitative Blood Count (QBC), Blood

As a major distributor and supplier of medical testing equipment, Diagnostic Automation/Cortez Diagnostics, Inc. offers two convenient, reliable, and inexpensive Malaria Rapid Tests: One Malaria Rapid Test is for Pan-LDH antigens. This Malaria Rapid Test can detect all four species of malaria Plasmodium falciparum, Plasmodium vivax, Plasmodium oval, and Plasmodium malaria Rapid Diagnostic Test (RDT) RDT is a device that can detect malaria antigen in a small amount of blood (5μl) by immunochromatographic assay (colour change in an absorbing nitrocellulose strip) with monoclonal antibodies directed against the parasite antigen Malaria is the most common single diagnosis made in many countries in Africa. Microscopy is the gold standard for laboratory diagnosis of malaria parasite, but it requires adequate training and the time to get results is longer than that for Rapid Diagnostic Tests (RDTs). Use of RDTs is an alternative diagnostic method. This method is quick and easy to carry out

2. Malaria Rapid Diagnostic Tests. Malaria RDT employ lateral flow immunochromatographic technology similar to rapid pregnancy tests. In these assays, the clinical sample migrates as a liquid across the surface of a nitrocellulose membrane by capillary action [13, 27, 33, 34].For a given targeted parasite antigen, two sets of monoclonal or polyclonal antibodies are used, a capture antibody and. 2.2 Standard Operating Procedures in malaria diagnosis 5 2.2.1 Broad structural components of SOP 6 Chapter 3 Current status of malaria microscopy and quality assurance 7-8 3.1 Microscopy 7 laboratory diagnosis involves microscopy, rapid diagnostic tests and quality management Test Performance Characteristics of the Rapid Diagnostic Test (RDT) versus Blood Smear for Malaria Diagnosis For nonfalciparum malaria (ie, malaria due to Plasmodium vivax, Plasmodium ovale , or Plasmodium malariae ), the sensitivity of the RDT was lower, at 86% (18 of 21 specimens), which was comparable to the 76% sensitivity (16 of 21) for. One Step Malaria pf (HRPⅡ)/PANLDH Antigen Detection Complete Test Kit (Whole Blood) Intended use: For the rapid qualitative determination of Malaria P. falciparum specific histidine rich protein-2 (Pf HRP-2)and malaria PANLDH specific lactate in human blood as an aid in the diagnosis of Malaria infection

of malaria rapid diagnostic tests There is increasing demand for countries to improve malaria diagnosis in view of wide-scale introduction of expensive antimalarial medicines and the decreasing malaria trends in many countries. Therefore, guidance is required for selecting rapid diagnostic tests (RDTs) for malaria that meet quality standards As more and more countries approaching the goal of malaria elimination, malaria rapid diagnostic tests (RDT) was recomendated to be a diagnostic strategy to achieve and maintain the statute of malaria free, as it's less requirments on equipment and experitise than microscopic examination. But there are very few economic evaluations to confirm whether RDT was cost-effective in the setting of. Rapid diagnostic tests have been of tremendous help in malaria control in endemic areas, helping in diagnosis and treatment of malaria cases. It is heavily relied upon in many endemic areas where microscopy cannot be obtained. However, caution should be taken in the interpretation of its result in clinical setting due to its limitations and inherent weakness Surveillance and diagnosis of Plasmodium falciparum malaria relies predominantly on rapid diagnostic tests (RDT). However, false-negative (FN) RDT results are known to occur for a variety of reasons, including operator error, poor storage conditions, pfhrp2/3 gene deletions, poor performance of specific RDT brands and lots, and low-parasite density infections

CDC - Malaria - Diagnosis & Treatment (United States

Rapid Testing. In early 2010, WHO recommended that every suspected malaria case be confirmed by microscopy or an RDT prior to treatment. In recent years, the availability of high-quality, inexpensive RDTs has made it possible to significantly improve and expand diagnostic testing across all levels of the health system, from district hospitals to community-based programmes. Context Improving the accuracy of malaria diagnosis with rapid antigen-detection diagnostic tests (RDTs) has been proposed as an approach for reducing overtreatment of malaria in the current era of widespread implementation of artemisinin-based combination therapy in sub-Saharan Africa.. Objective To assess the association between use of microscopy and RDT and the prescription of antimalarials

Malaria Parasite Test Procedure, Principle, Results

  1. al pain after visiting friends and relatives in India. While in India, the individual was not ill and had no signs or symptoms of malaria
  2. A rapid malaria test kit that could aid diagnosis in developing countries. A close-up of a prototype of the NTU-developed rapid malaria test kit. Credit: NTU Singapore. A team of scientists from.
  3. AdvDx Malaria Pf Rapid Malaria Ag Detection Test (with Sample applicator, Alcohol Swab, Lancet, Buffer solution) Advy Chemical Pvt Ltd Plot No.A-334,336,338 & A-337 & 339, Road no. 25 & 26, Wagle industial Estate, Thane 400 604, INDIA Cassette 25 24 2-400C WHO PQ WHO PQ Version 29 29 June 2021 LIST OF RAPID DIAGNOSTIC TEST (RDT) KITS FOR MALARIA
  4. NONMICROSCOPIC RAPID DIAGNOSTIC TESTS. A recent congress of the WHO produced a document entitled New Perspectives in Malaria Diagnosis (WHO/MAL/2000.1091) . Certain recommendations were presented on what nonmicroscopic rapid diagnostic tests (RDT) should provide
  5. imize overuse and thereby delay development of resistance to artemisinin-based combination therapies (ACTs)
  6. ation of blood, utilizing blood films. Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens. More recently, modern techniques utilizing antigen tests or polymerase chain reaction have been discovered, though these are not widely.
  7. The one step Cassette Style Malaria P.f. test is a rapid test based on the principle of immunoassay combined with conjugated colloid gold technology. The Malaria P.f. test((Whole Blood) is a diagnostic device to detect the presence of the P.f. antigen in whole bloo

RDTs for Malaria Diagnosis: Principle, Results, Advantages

malaria rapid diagnostic tests (mRDTs) available for people of all ages. According to data from the Zanzibar Malaria Elimination Program (ZAMEP), 300,892 mRDTs were used in Zanzibar in 2015. Overall prevalence is defined as the total number of cases divided by the total population gens currently targeted by rapid diagnostic tests (RDT) are histidine-rich proteins 2 (HRP-2), Plasmodium lactate dehy-drogenase (PLDH) and Plasmodium aldolase.7 Rapid diagnostic tests such as dipstick format kits for the detection of malaria antigen are now commercially avail-able. In recent years multiple studies have found that rapid

BinaxNOW Malaria Abbott Point of Care Testin

Lee SH, Kara UA, Koay E, Lee MA, Lam S, Teo D. New strategies for the diagnosis and screening of malaria. Int J Hematol 2002;76(suppl 1):291-293. Moody A. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 2002;15:66-78. Hanscheid T, Grobusch MP: How useful is PCR in the diagnosis of malaria? Trends Parasitol 2002;18:395-39 Malaria Pf/Pv is a rapid, qualitative immunoassay for the detection of P. falciparum specific histidine rich protein -2 (Pf HRP-2) and Pv malaria specific Parasite lactate dehydrogenase (pLDH) in human blood as an aid in the diagnosis of Malaria infection This test also used to differentiate the P. falciparu

Malaria Rapid Tests RDT Available for Online Order. BinaxNOW & SD BIOLINE Supplied by Abbot Malaria: Procedure: Rapid Diagnostic Test: Phase 4: Detailed Description: In many settings the majority of people with malaria particularly the poorest do not access formal care but access anti-malarials at the informal community level. ACTs were previously unaffordable to this group but this should change with the introduction of the AMFm Reports of human infection with Plasmodium knowlesi, a monkey malaria, suggest that it and other nonhuman malaria species may be an emerging health problem. We report the use of a rapid test to supplement microscopic analysis in distinguishing the 5 malaria species that infect humans

RDTs for Malaria Diagnosis: Principle, Results andWHO | How malaria RDTs work

A review of malaria diagnostic tools: microscopy and rapid

detecting rapid diagnostic tests (RDTs) forms a vital part of this strategy, forming the backbone of expansion of access to malaria diagnosis as they provide parasite-based diagnosis in areas where good quality microscopy cannot be maintained. The number of RDTs available, and the scale of their use, has rapidly increased over the past few years The test procedure, precautions and interpretation of results for this test must be followed when testing. • Anti-coagulants such as heparin, EDTA, and citrate do not affect the test result. • Do not mix reagent of different lots. • The test is limited to the detection of antigen to Malaria Plasmodium sp. Although the test is very accurate i 2. As with all diagnostic tests, the result must be correlated with clinical findings. If the test result is negative and malaria infection suspicion still exists, additional follow-up testing using other clinical methods is recommended. 3. A negative result at any time does not preclude the possibility of an early malaria infection. test P.f. P.f Malaria rapid diagnostic tests (RDTs) are a relatively new and evolving technology, which can provide parasite-based diagnosis in remote areas where microscopy is difficult to support and where.

[Rapid diagnostic test for malaria

  1. Advice for healthcare workers on malaria prophylaxis is available via the National Travel Health Network and Centre website: www.nathnac.org. 1.2 Key Points Test for malaria in all travellers returning from the tropics with a fever Confirm a positive malaria dipstick rapid diagnostic test with a blood fil
  2. Last updated on June 1st, 2021 Rapid diagnostic tests or RDTs for malaria are a way to test whether a person with malaria like symptoms actually has malaria. Malaria parasites produce proteins called antigens. RDT
  3. Wongsrichanalai C, Barcus MJ, Muth S, Sutamihardja A, Wernsdorfer WH. A review of malaria diagnostic tools: microscopy and rapid diagnostic test (RDT). Am J Trop Med Hyg. 2007 Dec. 77(6 Suppl):119-27
  4. evidence of reduction of malaria cases and deaths in Rwanda and 28. Zikusooka CM, McIntyre D, Barnes KI: Should countries implementing an Ethiopia due to rapid scale-up of malaria prevention and treatment. artemisinin-based combination malaria treatment policy also introduce Malar J 2009, 8:14. rapid diagnostic tests

Malaria Rapid Diagnostic Tests Clinical Infectious

  1. Malaria Antigen Detection• Immunologic assays to detect specific antigens• Commercial kits now available as immunochromatographic rapid diagnostic tests (RDTs), used with blood • P. falciparum histidine-rich protein 2 (PfHRP-2) • parasite LDH (pLDH)• Monoclonal and polyclonal antibodies used in antigen (Ag) capture test• Species.
  2. Malaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 5 (2013). 1.Diagnostic Techniques and Procedures. 2.Malaria - diagnosis. 3.Diagnostic Tests, Routine - methods. I.World Health Organization. ISBN 978 92 4 150755 4 (NLM classification: WC 750
  3. Rapid diagnostic tests have greatly improved malaria treatment in the last decade, but they also had some unexpected bad consequences, a large new study has found.. As hoped, the tests — which.
  4. g and requires training because of the procedures involved, furthermore it's expensive because of the rea-gents and complex equipment required to run the assay [7]. The introduction of malaria rapid diagnostic tests (RDTs) has enhanced quick exa
  5. Maltha et al., Malaria rapid diagnostic tests in travel medicine, in this issue). For all species, RDT sensitivity declines at lower parasite density [34,44,45]. There is much heterogeneity among reported results for RDT diagnostic accuracy, which may depend on several factors: the RDT product used [45-47], local epidemiolog
  6. Malaria mortality is mainly a direct consequence of inadequate and/or delayed diagnosis and case management. Some important control interventions (e.g. long-lasting insecticidal nests) have contributed to reduce malaria morbidity and mortality in different parts of the world. Moreover, the development and effective use of rapid diagnostic tests (RDTs) hold promise to further enhance the.
  7. Background. Diagnosing Plasmodium falciparum malaria during pregnancy is a great challenge for clinicians because of the low density of parasites in the peripheral blood and parasite sequestration in the placenta. Nevertheless, few data on the use of malaria rapid diagnostic test (RDT) during pregnancy have been published. Methods. P. falciparum infections were assessed in 156 febrile pregnant.
Malaria Antigen Test: Principle, Procedure, Results andSD Bioline Malaria Test kit - Medixab Nigeria Buy medicals

Evaluation of a rapid diagnostic test (CareStart™ Malaria

One Step Malaria pf (HRPⅡ)/PANLDH Antigen Detection Test Device(Whole Blood) Intended use: For the rapid qualitative determination of Malaria P. falciparum specific histidine rich protein-2 (Pf HRP-2)and malaria PANLDH specific lactate in human blood as an aid in the diagnosis of Malaria infection Background: Rapid diagnostic test (RDT) is a simpler, easy to read malaria diagnostic test. It was introduced by the World Health Organization (WHO) to supplement the use of microscopy and can be used alone in areas where microscopy is unavailable. Its introduction was necessary to maintain the WHO test-based treatment protocol for malaria, as dependence of microscopy which is the gold.

Treatment guided by rapid diagnostic tests for malaria in

The majority of Plasmodium falciparum malaria diagnoses in Africa are made using rapid diagnostic tests (RDTs) that detect histidine-rich protein 2. Increasing reports of false-negative RDT. Introduction: Malaria Rapid Diagnostic Test (MRDT) is widely used for diagnostic purposes in endemic areas and resource-constrained settings. However, concern about the accuracy of RDTs has made its wide-scale usage a debatable issue. Aim: To assess the validity of antigen detecting MRDT kits by comparing the test results with that of conventional Peripheral Smear (PS) examination used for.

Malaria Antigen Rapid Test - YouTube

Malaria rapid diagnostic tests - an implementation guide vii Introductory note This implementation guide is a step-by-step methodological approach to implementing the use of rapid diagnostic tests (RDTs) as a diagnostic tool in malaria control programmes Test Includes: Antigen testing for Plasmodium falciparum as well as pan-malarial antigen that is shared by other Plasmodium species causing human malaria. Logistics Test Indications: Useful for providing a rapid result in diagnosing malaria. Lab Testing Sections: Hematology Phone Numbers: MIN Lab: 612 -813 6280 STP Lab: 651-220-6550 Test. Malaria, Paracheck and Parasight F-test for detection of P. falciparum histidine-rich protein 2 [2, 3]. However, alternative human body fluids such as urine, which are obtained by less invasive procedures, are equally rich in malaria diagnostic antigens and could be used to produce more acceptable MAb-based tools for diagnosis of malaria Cross reaction of PfHRP2 with non-falciparum malaria could give false positive results for P. falciparum and mixed infections containing asexual stages of P. falciparum could be interpreted as negative in about one third of the patients. Comparison of Rapid Diagnostic Tests for Malaria Antigen The benefits of malaria rapid diagnostic test (mRDT) technologies are well documented [1, 2] and globally acknowledged.World Health Organisation (WHO) has endorsed mRDTs as adjunct tests to microscopy for parasitological confirmation of malaria in routine fever case management at lower levels of healthcare [].However, endorsing a new or improved health technology in itself does not guarantee. Malaria screening was done using microscopy method, Rapid Diagnostic Test and molecular assay. The microscopy method used was thick and thin blood film microscopy technique stained with Giemsa stain. Malaria Plasmodium falciparum antigen detection kit was used for Rapid Diagnostic Test while molecular Assay was carried out usin